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AQA · GCSE · Biology

Free AQA GCSE Biology
Practice Paper

8 mixed-difficulty practice questions in the style of real AQA GCSE papers — answers, mark-scheme-style explanations, and the official exam structure all on one page.

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What the real AQA GCSE Biology paper looks like

Paper 1
Approximately 1 hour 30 minutes, ~70-100 marks. Covers Topics 1-4 of the specification.
Paper 2
Approximately 1 hour 30 minutes, ~70-100 marks. Covers Topics 5-8 of the specification.
Paper 3
Where applicable — e.g. Combined Science, Languages. Includes synoptic and applied questions.
Total exam time: ~3 hours across two or three papers.
Grading: Grades: 9 (highest) to 1 (lowest), with U (ungraded). A grade of 4 is a standard pass; 5 is a strong pass.

Mini practice paper: 8 questions

Mixed-difficulty questions from across the Biology syllabus. Tap "Show answer" after each to check yourself.

Q1 · Difficulty 1/3

Which of the following best defines biodiversity?

  1. A) The total number of individuals in a single species
  2. B) The variety of all living organisms in an area, including different species and genes
  3. C) The number of producers in a food chain
  4. D) The variety of habitats found only in rainforests
Show answer & explanation
✓ Answer: BB) The variety of all living organisms in an area, including different species and genes
Biodiversity refers to the variety of all life on Earth, including the range of different species, genetic variation within species, and the variety of ecosystems. It is not limited to one species, one habitat type, or one trophic level.
Q2 · Difficulty 1/3

What is an allele?

  1. A different version of a gene
  2. A section of DNA that codes for a protein
  3. The complete set of genetic information in an organism
  4. A pair of chromosomes found in body cells
Show answer & explanation
✓ Answer: AA different version of a gene
An allele is a different version of a gene. For example, the gene for eye colour has alleles for brown eyes and blue eyes. Students often confuse alleles with genes themselves.
Q3 · Difficulty 1/3

Which of the following is the correct definition of osmosis?

  1. A. The movement of any molecule from high to low concentration through a membrane
  2. B. The net movement of water molecules from a region of higher water potential to lower water potential through a partially permeable membrane
  3. C. The movement of solute particles from low concentration to high concentration across a cell membrane
  4. D. The net movement of water molecules from low water potential to high water potential through a partially permeable membrane
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✓ Answer: BB. The net movement of water molecules from a region of higher water potential to lower water potential through a partially permeable membrane
Osmosis specifically describes the net movement of water molecules (not solute) across a partially permeable membrane, always from a region of higher water potential to lower water potential. Option D reverses the direction, which is a common student error.
Q4 · Difficulty 2/3

Which statement best explains why sexual reproduction produces offspring with more genetic variation than asexual reproduction?

  1. Sexual reproduction uses mitosis to generate genetically different daughter cells.
  2. Sexual reproduction involves more cell divisions than asexual reproduction.
  3. Sexual reproduction combines genetic material from two parents via meiosis and fertilisation.
  4. Sexual reproduction introduces mutations at a higher rate than asexual reproduction.
Show answer & explanation
✓ Answer: CSexual reproduction combines genetic material from two parents via meiosis and fertilisation.
Sexual reproduction combines genetic material from two genetically different parents; meiosis introduces further variation through crossing over and independent assortment, and fertilisation randomly combines gametes. The number of cell divisions is not the source of genetic variation. Mutation rate is not inherently higher in sexual reproduction. Mitosis produces genetically identical cells — it is meiosis that generates diversity.
Q5 · Difficulty 2/3

Which of the following is an example of an in situ conservation method?

  1. A) Storing seeds in a seed bank
  2. B) Keeping endangered animals in a zoo breeding programme
  3. C) Protecting a species within its natural habitat as a nature reserve
  4. D) Growing rare plants in botanical gardens
Show answer & explanation
✓ Answer: CC) Protecting a species within its natural habitat as a nature reserve
In situ conservation means protecting species within their natural habitats, such as through nature reserves or national parks. Seed banks, zoos, and botanical gardens are ex situ methods because the organisms are conserved outside their natural environment.
Q6 · Difficulty 2/3

A student investigates osmosis using dialysis tubing filled with concentrated sugar solution. The tubing is placed in a beaker of distilled water. Which observation would be expected after one hour?

  1. A. The dialysis tubing decreases in mass as sugar leaves through the tubing
  2. B. The dialysis tubing increases in mass as water enters by osmosis
  3. C. The dialysis tubing stays the same mass because osmosis requires energy
  4. D. The dialysis tubing increases in mass as sugar draws in water by active transport
Show answer & explanation
✓ Answer: BB. The dialysis tubing increases in mass as water enters by osmosis
Dialysis tubing acts as a partially permeable membrane. Water moves by osmosis from the high water potential distilled water into the lower water potential sugar solution inside the tubing, increasing its mass. Sugar molecules are too large to pass through the membrane.
Q7 · Difficulty 3/3

A potato cylinder of initial mass 8.0 g is placed in a sucrose solution and after 40 minutes has a mass of 8.6 g. A second cylinder of initial mass 10.0 g is placed in the same solution and gains 0.6 g. Which cylinder shows a greater percentage change in mass?

  1. A. The first cylinder, because it gained 0.6 g just like the second
  2. B. The second cylinder, because it had a larger initial mass
  3. C. The first cylinder, because its percentage change is higher
  4. D. Both cylinders show exactly the same percentage change
Show answer & explanation
✓ Answer: CC. The first cylinder, because its percentage change is higher
Cylinder 1: percentage change = (0.6/8.0) × 100 = 7.5%. Cylinder 2: percentage change = (0.6/10.0) × 100 = 6.0%. Although both gained the same absolute mass, cylinder 1 shows a greater percentage change. This is why percentage change, not absolute change, must be used when comparing samples of different starting masses.
Q8 · Difficulty 3/3

A student claims that intensive farming methods, such as keeping animals indoors and limiting their movement, increase the efficiency of biomass transfer to humans. Evaluate this claim.

  1. The claim is incorrect because animals indoors cannot photosynthesise efficiently
  2. The claim is correct because less energy is lost to respiration, so more biomass is retained for human consumption
  3. The claim is incorrect because animals indoors eat more food, increasing biomass loss
  4. The claim is correct because indoor animals are at a lower trophic level than outdoor animals
Show answer & explanation
✓ Answer: BThe claim is correct because less energy is lost to respiration, so more biomass is retained for human consumption
Limiting movement and controlling temperature reduces the energy animals use for respiration, meaning more of the biomass they consume is converted into body mass rather than lost as heat. This increases the efficiency of biomass transfer to humans. However, this raises significant ethical concerns about animal welfare.
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AQA GCSE Biology FAQ

What does the AQA GCSE Biology exam look like?
The AQA GCSE Biology exam is structured across 3 components. Paper 1: Approximately 1 hour 30 minutes, ~70-100 marks. Covers Topics 1-4 of the specification. Paper 2: Approximately 1 hour 30 minutes, ~70-100 marks. Covers Topics 5-8 of the specification. Paper 3: Where applicable — e.g. Combined Science, Languages. Includes synoptic and applied questions. Total exam time: ~3 hours across two or three papers.
Can I download a free AQA GCSE Biology past paper?
Real AQA past papers are published directly by AQA on their official website. Kramizo doesn't redistribute copyrighted past papers, but we do generate free AI-written practice papers in the exact same style — same command words, same difficulty tier, same mark conventions. Use this practice paper as warm-up, then time yourself on official past papers before exam day.
How is AQA GCSE Biology graded?
Grades: 9 (highest) to 1 (lowest), with U (ungraded). A grade of 4 is a standard pass; 5 is a strong pass. Kramizo's practice questions are tagged with difficulty 1-3 mapping roughly to the lower, middle, and top grade boundaries you'll encounter in the real exam.