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AQA GCSE·🧬 Biology·higher

AQA GCSE Biology — Paper 1 (Higher Tier)

105 minutes📊 100 marks📄 Paper 1 (Higher Tier)
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ℹ️ About this paper: This is an exam-board-aligned practice paper written in the style of AQA GCSE — not an official past paper. Use it for timed practice, then check against the mark scheme included below. For official past papers, see the exam board's website.
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AQA GCSE Biology — Paper 1 (Higher Tier)

Total marks: 100 · Duration: 105 minutes · Tier: Higher

Instructions to candidates

  • Answer all questions in the spaces provided.
  • Use a black ball-point pen. You may use an HB pencil for graphs and diagrams.
  • A calculator may be used.
  • For Section A, answer all questions. For Section B, answer both extended response questions fully.
  • Write your answers clearly and in good English. Marks may be deducted for poor spelling, punctuation and grammar.

Paper

Section A — Structured Questions (60 marks)


Question 1

A student investigated the effect of pH on the rate of enzyme action. They used the enzyme amylase to break down starch. The student tested the enzyme at different pH values and recorded the time taken for all the starch to be broken down.

[Table 1: Results of enzyme investigation]

pH Time taken for starch to be broken down (s) Rate of reaction (1/time) (s⁻¹)
3 240 0.0042
5 180 0.0056
7 90
9 210 0.0048
11 360 0.0028

(a) Calculate the rate of reaction at pH 7. Give your answer to 4 decimal places. (2 marks)

(b) Describe the effect of pH on the rate of enzyme action shown in Table 1. (2 marks)

(c) Explain why the rate of reaction decreases at pH 11. (3 marks)

(d) The student kept the investigation at 25°C throughout. Suggest why temperature control is important in this investigation. (2 marks)


Question 2

Figure 1 shows a plant cell and an animal cell as seen under a light microscope.

[Figure 1 shows two diagrams: Cell A (plant cell) showing cell wall, cell membrane, cytoplasm, nucleus, vacuole, and chloroplasts. Cell B (animal cell) showing cell membrane, cytoplasm, nucleus, and mitochondria]

(a) State two structures visible in Cell A that are not present in Cell B. (2 marks)

(b) Mitochondria are not shown in Cell A. Explain why mitochondria are present in plant cells even though they contain chloroplasts. (3 marks)

(c) A student observes Cell A using a light microscope with a magnification of ×400. The actual length of the cell is 50 μm. Calculate the length of the cell image in mm. (3 marks)

(d) Electron microscopes have much higher resolution than light microscopes. Explain what is meant by resolution and why this is useful when studying cells. (3 marks)


Question 3

Cancer is caused by uncontrolled cell division. Stem cells also divide many times.

(a) State two differences between cancer cells and stem cells. (2 marks)

(b) Describe what happens during mitosis. (4 marks)

(c) Scientists have developed cancer treatments that stop cells from dividing. Explain why these treatments can cause side effects such as hair loss and poor immune function. (4 marks)


Question 4

Figure 2 shows the digestive system.

[Figure 2 shows a diagram of the human digestive system with parts labeled A, B, C, D, and E. A = salivary glands, B = liver, C = stomach, D = pancreas, E = small intestine]

(a) Name organ D. (1 mark)

(b) Lipase is produced by organ D. State two other organs that produce lipase. (2 marks)

(c) The stomach produces hydrochloric acid. Give two reasons why the stomach produces hydrochloric acid. (2 marks)

(d) The small intestine is adapted for absorption of digested food molecules. Describe three adaptations of the small intestine that increase the rate of absorption. (3 marks)


Question 5

Students investigated osmosis using potato cylinders. They placed potato cylinders of equal mass into different concentrations of sugar solution for 30 minutes. The students measured the mass of each potato cylinder before and after placing them in the solutions.

[Table 2: Results]

Concentration of sugar solution (mol/dm³) Mass at start (g) Mass at end (g) Change in mass (g) Percentage change in mass (%)
0.0 2.5 2.9 +0.4 +16.0
0.2 2.5 2.7 +0.2 +8.0
0.4 2.5 2.5 0.0 0.0
0.6 2.5 2.3 -0.2
0.8 2.5 2.1 -0.4 -16.0

(a) Calculate the percentage change in mass for the potato cylinder in 0.6 mol/dm³ sugar solution. (2 marks)

(b) Explain why the potato cylinder in 0.0 mol/dm³ solution increased in mass. (3 marks)

(c) Use the results to estimate the concentration of sugar solution inside the potato cells. Explain your answer. (2 marks)

(d) The students used potato cylinders of equal mass at the start. Suggest one other variable the students should have controlled. (1 mark)


Question 6

The human circulatory system transports substances around the body.

(a) The heart is described as a double pump. Explain what this means. (2 marks)

(b) Figure 3 shows the heart.

[Figure 3 shows a diagram of the heart with four chambers and blood vessels. Structure X is labeled pointing to the left ventricle wall]

Explain why structure X has a thicker wall than the other chambers. (2 marks)

(c) Red blood cells are adapted to carry oxygen. Describe two adaptations of red blood cells and explain how each adaptation helps the cell carry oxygen efficiently. (4 marks)

(d) People with coronary heart disease may have a stent fitted. Evaluate the use of stents and statins in treating coronary heart disease. (4 marks)


Section B — Extended Response (40 marks)


Question 7

A pharmaceutical company is developing a new drug to treat bacterial infections. Before the drug can be used to treat patients, it must go through extensive testing.

The company completes the following stages:

  • Computer modelling and testing on human cells in the laboratory
  • Testing on animals (mice and rabbits)
  • Clinical trials on human volunteers (healthy volunteers first, then patients with bacterial infections)
  • Peer review and publication of results

Some people argue that animal testing should be banned. Others argue it is essential for developing safe medicines.

Evaluate the use of animals in the development of new medicines. In your answer, you should:

  • discuss the reasons why animals are used in drug development
  • consider the ethical concerns about animal testing
  • evaluate whether alternative methods could replace animal testing. (10 marks)

Question 8

Transport systems in plants and animals show both similarities and differences in structure and function.

Plants have xylem and phloem tissue to transport substances. Animals have blood vessels including arteries, veins, and capillaries.

Compare the structure and function of transport systems in plants and animals. In your answer, you should refer to:

  • the substances transported in each system
  • the structure of the transport vessels
  • how the transport systems are adapted for their functions. (10 marks)

Question 9

Figure 4 shows a pyramid of biomass for a food chain in a woodland ecosystem.

[Figure 4 shows a pyramid with four levels:

  • Top: Owls (0.5 kg/m²)
  • Third: Small birds (2 kg/m²)
  • Second: Caterpillars (8 kg/m²)
  • Bottom: Oak tree leaves (40 kg/m²)]

(a) Calculate the percentage efficiency of biomass transfer from caterpillars to small birds. (2 marks)

(b) Explain why biomass decreases at each stage of the food chain. (4 marks)

(c) Organic farmers use biological control methods rather than pesticides to control pests. An organic farmer introduces ladybirds to control aphids on crops.

Evaluate the use of biological control compared to pesticides for controlling crop pests. In your answer, you should consider:

  • the effectiveness of each method
  • the impact on the environment
  • economic factors. (6 marks)

Question 10

Scientists investigated the effect of light intensity on the rate of photosynthesis in a water plant called Elodea. They counted the number of oxygen bubbles produced per minute at different distances from a light source.

[Table 3: Results]

Distance from light (cm) Number of bubbles per minute Light intensity (arbitrary units)
10 48 100
20 24 25
30 11 11
40 6 6
50 4 4

(a) Describe the relationship between light intensity and the rate of photosynthesis shown in Table 3. (2 marks)

(b) Explain why increasing light intensity increases the rate of photosynthesis. (3 marks)

(c) The scientists maintained the temperature at 25°C and used air enriched with carbon dioxide.

Explain why these factors were controlled and how each could become a limiting factor for photosynthesis. (6 marks)

(d) Commercial greenhouses use artificial lighting and heaters to increase crop yield.

Evaluate whether the increased costs of using artificial lighting and heating in greenhouses are justified by the increased crop yields. In your answer you should consider:

  • how these factors affect photosynthesis
  • the costs involved
  • the economic benefits. (7 marks)

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