Mark Scheme
Section A — Structured Questions (54 marks)
Question 1
(a) Distance from lamp / light intensity (1)
Accept: how far the lamp is from the pondweed
(b) Any one from:
- Temperature (of water)
- Type / species of pondweed
- Volume / amount of water
- Same piece of pondweed used
- Concentration of carbon dioxide
- Time period for counting (1)
Accept: mass of pondweed
(c) Any two from:
- Bubbles may be different sizes / volumes (1)
- Some (oxygen) gas may dissolve in water / not form bubbles (1)
- Bubbles may be carbon dioxide / not oxygen (1)
- Difficult to count bubbles accurately (if produced quickly) (1)
Maximum 2 marks
(d)
45 - 7 = 38 (1)
(38 ÷ 45) × 100 = 84.4% / 84% (1)
Accept 84 - 85%
Award 1 mark for correct working even if final answer incorrect
(e) Any three from:
- Place tube / beaker in water bath (1)
- Use different / range of temperatures (1)
- Keep lamp at same / fixed distance (1)
- Measure rate of photosynthesis at each temperature / count bubbles (1)
- Allow time for pondweed to reach each temperature / equilibrate (1)
Maximum 3 marks
Question 2
(a)
W: Aorta (1)
X: Left ventricle (1)
Both required for both marks
(b) Any two from:
- (Left ventricle / X) pumps blood around the (whole) body / to the body (1)
- (Right atrium / Z) receives blood from the body / vena cava (1)
- (Left ventricle / X) needs to generate higher pressure (1)
- (Left ventricle / X) needs thicker muscle (to generate higher pressure) (1)
Maximum 2 marks
Accept: left ventricle pumps blood further
(c) Any three from:
- Brain / medulla detects change (1)
- (Impulses sent) by nerves / nervous system (1)
- To the heart / SAN / pacemaker (1)
- (Impulses cause) heart to beat faster / increase heart rate (1)
- This increases oxygen / glucose supply (to muscles) (1)
Maximum 3 marks
(d) Any two from:
- (Natural) pacemaker / SAN not working (properly) (1)
- Heart beating irregularly / arrhythmia (1)
- Heart beating too slowly / bradycardia (1)
- Heart rate too low / insufficient blood being pumped (1)
Maximum 2 marks
Question 3
(a) ff (1)
Reject: Ff or FF
(b) Any two from:
- Both parents are carriers / heterozygous (1)
- Both parents have genotype Ff (1)
- Each parent passes on the recessive allele / f (1)
- Child has genotype ff / two recessive alleles (1)
Maximum 2 marks
Accept: child inherits one f from each parent
(c)
Genetic diagram showing:
- Parents' genotypes clearly identified as Ff × Ff (1)
- Gametes identified as F and f for both parents (1)
- Offspring genotypes shown: FF, Ff, Ff, ff (1)
- Correct method shown (Punnett square or other recognised method) (1)
Accept alternative but correct genetic diagram formats
Example acceptable diagram:
Parents: Ff × Ff
Gametes: F f F f
Offspring: FF Ff Ff ff
(d)
1 in 4 / 25% / 0.25 (2)
Award 1 mark for 1 in 4 or 1/4 without conversion to percentage
Award 2 marks for correct answer with correct working
Question 4
(a)
Diffusion / movement of water (molecules) (1)
From high / higher water potential to low / lower water potential / down a water potential gradient (1)
Through a partially / selectively / semi-permeable membrane (1)
Maximum 2 marks
Accept: from dilute to concentrated solution
Reject: movement of sugar
(b) 0.4 mol/dm³ (1)
Accept: between 0.3 and 0.5
(c) Any three from:
- Water potential of solution lower / more negative than (water potential of) potato (cells) (1)
- Water moves out of potato cells (1)
- By osmosis (1)
- (Cells lose water and) become flaccid / plasmolysed (1)
- (Causing) decrease in length / volume (1)
Maximum 3 marks
(d) Any two from:
- Boiling damages / denatures / destroys cell membranes (1)
- Membrane no longer partially permeable / selectively permeable (1)
- Osmosis cannot occur / water can move freely (in and out) (1)
- Cells are dead / non-functional (1)
Maximum 2 marks
Question 5
(a)(i) Blue-black / black (1)
Accept: dark blue, purple
(a)(ii) Brown / orange / yellow / remains (brown) (1)
Accept: no colour change
(b) pH 7 / 7 (1)
(c) Any three from:
- (pH change) changes the shape of the active site (1)
- Active site becomes denatured (1)
- Substrate no longer fits (into active site) (1)
- Enzyme-substrate complex cannot form / fewer collisions between enzyme and substrate (1)
- (So) fewer successful collisions (per unit time) (1)
Maximum 3 marks
Reject: enzyme is destroyed / killed
Accept: enzyme is less effective
(d)
Rate = 1 ÷ 60 (1)
= 0.0167 / 0.017 s⁻¹ / per second (1)
Both required for full marks
Award 1 mark for correct calculation without unit
(e) Any two from:
- Temperature (of solutions) (1)
- Volume / concentration of starch solution (1)
- Volume / concentration of amylase solution (1)
- Volume / concentration of buffer solution (1)
- Time interval between tests (1)
Maximum 2 marks
Question 6
(a) Photosynthesis (1)
(b) Any two from:
- Animals eat / consume plants (1)
- (Carbon compounds) pass along food chain (1)
- Animals digest / break down carbon compounds (from plants) (1)
- (Animals) use carbon compounds for growth / respiration (1)
Maximum 2 marks
(c) Respiration (1)
(d) Any three from:
- Decomposers break down / decay dead organisms / waste products (1)
- (Decomposers) use carbon compounds in dead material (1)
- (Decomposers) respire / carry out respiration (1)
- (Respiration) releases carbon dioxide (into atmosphere) (1)
- Decomposers are bacteria / fungi (1)
Maximum 3 marks
(e) Any two from:
- Burning fossil fuels (1)
- Deforestation / cutting down trees (1)
- Increased respiration (from larger population) (1)
- Intensive farming / agriculture / livestock farming (1)
- Increased transport / industry / manufacturing (1)
Maximum 2 marks
Accept specific examples: coal, oil, gas for first marking point
Section B — Extended Response (36 marks)
Question 7
(a) Indicative content:
- Blood glucose rises after eating (shown in Figure 5)
- Glucose absorbed from digested food / carbohydrates
- Detected by pancreas
- Pancreas releases insulin
- Insulin causes glucose to be taken up by cells / converted to glycogen
- Stored in liver / muscles
- Blood glucose decreases back to normal / 80 mg/100 cm³
- When blood glucose too low, pancreas releases glucagon
- Glucagon causes glycogen to be converted back to glucose
- Released from liver into blood
- Negative feedback mechanism maintains constant level
Marking:
Award 1 mark for each valid point, up to maximum of 6 marks
Credit references to Figure 5 and Table 4
Credit explanation of hormonal control mechanism
Credit correct sequence of events
(b) Level 3 (7–9 marks):
A comprehensive discussion that covers causes, symptoms and treatment in detail. The response demonstrates thorough understanding of Type 1 diabetes with accurate scientific terminology throughout. Links between different aspects are clearly explained. All three aspects (causes, symptoms, treatment) are covered in depth.
Level 2 (4–6 marks):
A reasonable discussion that covers at least two of the three aspects (causes, symptoms, treatment). The response demonstrates sound understanding with generally accurate terminology. Some aspects may lack detail or clarity. Links may be present but not fully developed.
Level 1 (1–3 marks):
A limited discussion with basic statements about Type 1 diabetes. May focus on only one aspect. Scientific terminology may be limited or inaccurate. Little or no linking of ideas. Response may be list-like.
0 marks: No relevant content
Indicative content:
Causes:
- Autoimmune disease / immune system attacks beta cells
- Pancreas unable to produce (sufficient) insulin
- Genetic factors / inherited tendency
- Environmental triggers
- Usually develops in childhood / early life
Symptoms:
- High blood glucose / hyperglycaemia
- Glucose in urine
- Excessive thirst
- Frequent urination
- Weight loss
- Tiredness / fatigue
- Blurred vision
- Slow healing wounds
Treatment:
- Regular insulin injections / insulin therapy
- Cannot take insulin orally (because it would be digested)
- Blood glucose monitoring / testing
- Carbohydrate controlled diet
- Regular exercise
- Insulin pump (may be used)
- Must balance insulin dose with food intake and exercise
- No cure / requires lifelong management
Quality of extended response (3 marks):
- 3 marks: Answer is coherent, well-structured and logically sequenced. Specialist terminology used accurately throughout. Minimal errors in grammar, punctuation and spelling.
- 2 marks: Answer has reasonable structure and sequence. Specialist terminology generally used appropriately. Few errors in grammar, punctuation and spelling.
- 1 mark: Answer has some structure. Some use of specialist terminology. Frequent errors in grammar, punctuation and spelling.
- 0 marks: Answer lacks structure. Little or no specialist terminology. Poor grammar, punctuation and spelling.
Question 8
(a) Any four from:
- Camel loses less water (per day) than cow / 5 litres compared to 40 litres (1)
- Camel loses less water per kg body mass / 0.01 compared to 0.08 litres/kg (1)
- Camel loses 8 times less water per kg (1)
- (This means) camel needs to drink less frequently (1)
- Camel can survive longer without water / where water is scarce (1)
- Reference to numerical data from Table 5 (1)
Maximum 4 marks
(b) Any four from (must include two adaptations with explanations):
Large body size / surface area to volume ratio:
- Large body size / small surface area to volume ratio (1)
- Reduces (rate of) heat gain / water loss (1)
Long eyelashes:
- Keep sand out of eyes (1)
- Protection from sandstorms / desert conditions (1)
Wide feet:
- Spreads weight / prevents sinking (1)
- (Allows camel to) walk on sand (1)
Hump containing fat:
- Fat store / energy store (1)
- Can be respired to release energy / water (1)
- Allows survival when food / water scarce (1)
- Fat not distributed around body (so heat can be lost more easily) (1)
Maximum 4 marks (must be two adaptations, each with at least one mark)
(c) Level 3 (7–9 marks):
A comprehensive evaluation covering structural adaptations of desert plants, explanation of how these reduce water loss, and effective comparison with plants in wet conditions. Response is detailed with accurate scientific terminology. Links between structure and function are clearly explained. Comparative statements are developed and supported.
Level 2 (4–6 marks):
A reasonable evaluation covering most aspects. Structural adaptations described with some explanation of function. Some comparison made with plants in wet conditions. Terminology generally accurate but may lack some detail or development. Some links between structure and function present.
Level 1 (1–3 marks):
A limited evaluation with basic statements. May list structural features without clear explanation. Comparison with other plants limited or absent. Terminology basic or inaccurate. Limited links between structure and function.
0 marks: No relevant content
Indicative content:
Structural adaptations:
- Thick waxy cuticle
- Reduced leaves / spines / no leaves
- Swollen / fleshy stem
- Extensive / shallow root system
- Sunken stomata
- Stomata closed during day / open at night
- CAM photosynthesis
- Hairs / spines on surface
How adaptations reduce water loss:
- Thick cuticle reduces evaporation / waterproof layer
- Spines / reduced leaves reduce surface area for water loss
- Stem stores water
- Roots absorb water quickly when available / over wide area
- Sunken stomata trap water vapour / reduce water potential gradient
- Closing stomata (during day) prevents transpiration when hottest
- Hairs trap water vapour / create humid microclimate
Comparison with plants in wet conditions:
- Plants in wet conditions have thin cuticle / no waxy cuticle
- Large / broad leaves (to maximise photosynthesis)
- Stomata on surface / not sunken
- Stomata open during day
- Do not need to store water
- May have mechanisms to remove excess water / drip tips
- Different root systems / shorter roots
Evaluation points:
- Desert plants sacrifice photosynthesis rate for water conservation
- Structural adaptations are trade-off between water loss and gas exchange
- These adaptations allow survival where other plants cannot
- Adaptations evolved over time through natural selection
Quality of extended response (3 marks):
- 3 marks: Answer is coherent and logically structured. Uses specialist terminology accurately and appropriately throughout. Minimal errors in grammar, punctuation and spelling. Clear comparisons and evaluation present.
- 2 marks: Answer has some structure and logical sequence. Uses specialist terminology appropriately in most places. Few errors in grammar, punctuation and spelling. Some attempt at comparison or evaluation.
- 1 mark: Answer shows basic structure. Limited use of specialist terminology. Frequent errors in grammar, punctuation and spelling. Little or no comparison or evaluation.
- 0 marks: Answer lacks coherent structure. No appropriate specialist terminology. Poor grammar, punctuation and spelling that affects clarity.
Sample Answers with Examiner Commentary
Question 7(b) — Sample Answers
Grade 9 (top of Higher) answer
Type 1 diabetes is an autoimmune disease where the immune system attacks and destroys the beta cells in the pancreas that produce insulin. This means the pancreas cannot produce sufficient insulin to control blood glucose levels. There is a genetic component to Type 1 diabetes, as it tends to run in families, but environmental factors may also trigger the condition. It typically develops in childhood or early adulthood.
The symptoms of Type 1 diabetes result from high blood glucose concentration. The kidneys cannot reabsorb all the glucose, so glucose appears in the urine. This causes excessive urination and dehydration, leading to extreme thirst. Patients also experience unexplained weight loss because without insulin, cells cannot take up glucose for respiration, so the body breaks down fat and protein for energy instead. Other symptoms include tiredness due to lack of glucose in cells, blurred vision, and slow-healing wounds because high blood glucose affects blood circulation and immune function.
Treatment of Type 1 diabetes requires lifelong insulin therapy. Insulin must be injected (usually subcutaneously) because if taken orally it would be digested by proteases in the stomach. Patients need to regularly monitor their blood glucose levels using a finger-prick test or continuous glucose monitor. They must carefully balance their insulin doses with their carbohydrate intake and exercise levels. Eating carbohydrates raises blood glucose, so more insulin is needed, while exercise lowers blood glucose, so less insulin is required. Many patients use rapid-acting insulin before meals and slow-acting insulin to maintain baseline levels. Some patients use an insulin pump that delivers insulin continuously. Patients must also follow a healthy diet, controlling carbohydrate intake and eating regularly to avoid dangerous spikes or drops in blood glucose. There is currently no cure for Type 1 diabetes, so management is lifelong.
Mark: 9/9 + 3/3 QER = 12/12
Examiner commentary: This is an exemplary response demonstrating comprehensive understanding of all three aspects. The answer is exceptionally well-structured, covering causes (autoimmune, genetic, environmental triggers), symptoms (with clear explanations linking symptoms to underlying mechanism), and treatment (detailed, explaining why insulin is injected, the need for monitoring and balancing factors). Scientific terminology is used accurately throughout (beta cells, subcutaneously, proteases, continuous glucose monitor). The response shows clear understanding of cause-and-effect relationships and achieves full marks for quality of extended response due to excellent structure and specialist terminology.
Grade 6 (solid pass) answer
Type 1 diabetes is caused when the pancreas doesn't make enough insulin. This is because the body's immune system destroys the cells that make insulin. It can run in families and usually starts when people are young.
The symptoms include high blood sugar levels. This causes glucose to appear in urine which makes people need to go to the toilet a lot and feel very thirsty. People with Type 1 diabetes also lose weight because their cells can't use glucose for energy. They feel tired and may have blurred vision.
The treatment is insulin injections. Diabetics have to inject insulin because they can't take it as a tablet as it would get broken down in the digestive system. They need to check their blood sugar regularly using a blood test. They have to be careful about what they eat, especially sugary and carbohydrate foods. They need to match their insulin to their food and exercise. Exercise lowers blood sugar so they might need less insulin when they exercise. Some people have an insulin pump instead of injections. There is no cure so people have to manage their diabetes for their whole life.
Mark: 6/9 + 2/3 QER = 8/12
Examiner commentary: This is a solid mid-level response that addresses all three aspects (causes, symptoms, treatment) but with less depth and precision than the top answer. The candidate demonstrates sound understanding of the key concepts but misses some detail (e.g., doesn't mention beta cells specifically, doesn't name proteases, limited explanation of the autoimmune mechanism). The response would benefit from more scientific terminology (e.g., "subcutaneous," "hyperglycaemia," "rapid-acting/slow-acting insulin"). Structure is reasonable but could be more developed. Some good points made about balancing insulin with food and exercise. Quality of extended response achieves 2 marks due to reasonable structure and generally appropriate terminology, though with room for improvement.
Grade 3 (near miss) answer
Type 1 diabetes is when you don't have enough insulin in your body. Your pancreas stops working properly and can't make insulin anymore. It is genetic so you get it from your parents.
People with diabetes have high sugar levels in their blood. They feel thirsty and tired all the time. They also lose weight and need the toilet more. They might feel dizzy and unwell.
To treat diabetes you have to have insulin injections every day. You can't eat the insulin because your stomach acid would kill it. People with diabetes have to eat healthy food and not eat too much sugar or they will get more ill. They have to do blood tests to check their sugar levels. If they don't take their insulin they could die. Some people have a special machine that gives them insulin automatically.
Mark: 3/9 + 1/3 QER = 4/12
Examiner commentary: This response demonstrates basic understanding but lacks the detail, accuracy and development needed for higher marks. Key weaknesses include: oversimplification of cause (doesn't explain autoimmune mechanism), limited scientific terminology ("sugar" instead of "glucose," "stops working" rather than explaining beta cell destruction), misconception that it is simply inherited from parents without mentioning it's an autoimmune disease. Symptoms are mentioned but not explained (no link to mechanism). Treatment section shows some knowledge but lacks detail about insulin types, monitoring methods, and balancing factors. The phrase "stomach acid would kill it" shows misunderstanding (should explain enzymatic digestion). Structure is basic and list-like. To improve, this candidate needs to develop explanations, use correct scientific terminology, and make clear links between causes, symptoms and treatment mechanisms.
Question 8(c) — Sample Answers
Grade 9 (top of Higher) answer
Desert plants have evolved numerous structural adaptations that enable them to survive in hot, dry environments where water is scarce.
A key adaptation is the thick waxy cuticle covering all surfaces of the plant. This waterproof layer significantly reduces evaporation from the plant surface, minimizing water loss. In comparison, plants in wet conditions have a much thinner cuticle because water conservation is not a priority, and a thick cuticle would impede gas exchange needed for photosynthesis.
Desert plants typically have reduced leaves or modified leaves in the form of spines. Cacti, for example, have no leaves at all—the stem performs photosynthesis instead. This dramatically reduces the surface area available for water loss through transpiration. The spines also provide shade and create a boundary layer of still air around the plant, further reducing water loss. The fleshy, swollen stem serves as a water storage organ, allowing the plant to survive extended periods without rainfall. In contrast, plants in wet environments have large, broad leaves with extensive surface area to maximize light absorption and photosynthesis, as water loss is not a limiting factor. These plants have no need for water storage organs.
Another crucial adaptation is the presence of sunken stomata. In desert plants, stomata are located in pits or depressions, often surrounded by hairs. This creates a microenvironment with higher humidity, reducing the water potential gradient between the inside of the leaf and the atmosphere, thereby decreasing transpiration rate. Furthermore, many desert plants exhibit CAM (Crassulacean Acid Metabolism) photosynthesis, where stomata open at night when temperatures are lower and humidity is higher, absorbing carbon dioxide which is stored as malic acid. During the day, when water loss would be greatest, stomata remain closed and the stored carbon dioxide is released for photosynthesis. Plants in wet conditions have stomata on the leaf surface and keep them open during the day for gas exchange, as water is readily available.
Root adaptations are also significant. Many desert plants have either extensive shallow root systems that spread widely to capture water quickly after rare rainfall events, or extremely deep tap roots to access groundwater. By contrast, plants in wet conditions typically have less extensive root systems as water is readily available in the soil.
However, these adaptations come at a cost. By reducing leaf surface area and keeping stomata closed during the hottest parts of the day, desert plants significantly limit their rate of photosynthesis and therefore their growth rate. They grow much more slowly than plants in wet conditions. Nevertheless, these adaptations represent an effective evolutionary solution allowing plant survival in one of the most challenging environments on Earth. The structural features demonstrate the principle that natural selection favors characteristics that maximize survival in a specific environment, even if this means sacrificing growth rate and productivity.
Mark: 9/9 + 3/3 QER = 12/12
Examiner commentary: This is an outstanding response that comprehensively addresses all aspects of the question. The answer systematically covers multiple structural adaptations (cuticle, reduced leaves/spines, stem structure, stomata positioning, root systems) with clear explanations of how each reduces water loss. The candidate makes effective comparisons throughout with plants in wet conditions, showing genuine evaluative thinking. Excellent use of specialist terminology (transpiration, water potential gradient, CAM photosynthesis, malic acid, boundary layer). The response demonstrates deep understanding by explaining the trade-offs involved (reduced photosynthesis rate) and places adaptations in an evolutionary context. Structure is logical and coherent, with clear topic sentences and well-developed paragraphs. This achieves full marks for content and quality of extended response.
Grade 6 (solid pass) answer
Desert plants have many adaptations to help them survive in hot, dry conditions where there is very little water available.
One important adaptation is having a thick waxy cuticle on their surface. This stops water from evaporating from the plant, so the plant loses less water. Plants that live in wet places don't need such a thick cuticle because they have plenty of water available so it doesn't matter if they lose some.
Desert plants also have reduced leaves or spines instead of normal leaves. For example, cacti have spines instead of leaves. This reduces the surface area of the plant so less water can be lost through the surface. The stem of the cactus is thick and fleshy and stores water inside it for when there is no rain. Plants in wet conditions have large leaves because they need a big surface area for photosynthesis and they don't need to worry about water loss.
Another adaptation is having sunken stomata. The stomata are in pits which helps to reduce water loss because it traps moist air. Desert plants also close their stomata during the day when it is hot to stop water escaping, and open them at night when it is cooler. Normal plants have their stomata on the surface of the leaves and keep them open during the day for photosynthesis.
The roots of desert plants are often very long or spread out widely so they can collect water from a large area when it rains. They need to absorb water quickly because it doesn't rain very often in deserts.
These adaptations are very effective at reducing water loss and allowing desert plants to survive where other plants would die. However, having small leaves and closed stomata means desert plants can't do as much photosynthesis, so they grow more slowly than other plants.
Mark: 6/9 + 2/3 QER = 8/12
Examiner commentary: This is a competent response that covers the main structural adaptations and makes some comparative points. The candidate demonstrates sound understanding of key concepts (cuticle function, reduced leaves, sunken stomata, CAM-type behavior, root adaptations). Reasonable comparisons are made with plants in wet conditions. However, the response lacks some of the depth and sophistication of a top answer—for example, doesn't explain water potential gradient, doesn't use terms like "transpiration" consistently, doesn't mention CAM photosynthesis by name. Some explanations could be more precise (e.g., "moist air" rather than explaining the microenvironment and humidity gradient). The evaluative element at the end is present but brief. Structure is reasonable with clear paragraphs. Quality of extended response achieves 2 marks due to generally appropriate terminology and logical sequence, though scientific terminology could be more consistently accurate throughout.
Grade 3 (near miss) answer
Desert plants have lots of adaptations to survive in the desert where it is very hot and dry.
They have a waxy coating on them which stops water getting out. This is good because there isn't much water in the desert so they need to keep the water inside the plant. Plants in rainforests don't need this because they have lots of water.
Cacti don't have leaves, they have spikes instead. This means they lose less water. The spikes also protect them from animals that might try to eat them. The stem is fat and stores water inside like a water bottle. Other plants have normal leaves for photosynthesis.
Desert plants have their stomata inside holes so less water escapes. They close their stomata when it's hot so water doesn't evaporate. Other plants have stomata on the outside.
Desert plants have long roots to find water underground or roots that spread out to collect rain water. They have to collect water whenever it rains because it doesn't rain much in deserts.
All these adaptations mean desert plants don't lose much water and can survive in the desert heat. They are well adapted to their environment.
Mark: 3/9 + 1/3 QER = 4/12
Examiner commentary: This response shows basic understanding but lacks the detail, scientific accuracy and development needed for higher marks. Key weaknesses include: limited scientific terminology (uses "spikes" instead of spines, "waxy coating" rather than cuticle, "holes" instead of pits, doesn't use "transpiration"), oversimplified explanations without clear mechanisms (e.g., "stops water getting out" without explaining evaporation or transpiration), minimal comparative analysis (mentions rainforest once but doesn't develop comparison systematically), misses key adaptations (no mention of CAM photosynthesis or water storage in detail), no genuine evaluation of effectiveness or trade-offs. The final paragraph is too general and doesn't add substantive content. Structure is very basic with short paragraphs. To improve, the candidate needs to use correct scientific vocabulary, explain mechanisms more precisely, develop comparisons throughout, and provide genuine evaluation rather than simple statements. The response reads more like a list of facts than an integrated evaluation.