A student in Trinidad investigated translocation by analyzing sap from the phloem of a sugar cane stem. Which method would be most suitable for collecting pure phloem sap?

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Question

A student in Trinidad investigated translocation by analyzing sap from the phloem of a sugar cane stem. Which method would be most suitable for collecting pure phloem sap?

Accepted Answer

Using aphid stylets inserted into phloem tissue — Award 1 mark for B. Aphids naturally insert their stylets into sieve tubes. When the aphid is removed and the stylet left in place, pure phloem sap exudes due to pressure. A is incorrect because cutting releases sap from both xylem and phloem, plus other cell contents. C is incorrect because squeezing releases mixed cell contents, not pure phloem sap. D is incorrect because this would test water uptake, not phloem sap collection.

Answer

Cutting the stem and collecting the liquid that flows out

Answer

Placing the stem in water and collecting absorbed liquid

Answer

Squeezing the stem and collecting the juice

Answer

Using aphid stylets inserted into phloem tissue

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